dedicated complete medium cm-0251 Search Results


blood agar plate cultures  (Thermo Fisher)
99
Thermo Fisher blood agar plate cultures
Blood Agar Plate Cultures, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dedicated+complete+medium+cm-0251/AGAR/pmc00444807-196-6-11
Average 99 stars, based on 1 article reviews
blood agar plate cultures - by Bioz Stars, 2026-10
99/100 stars
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anti cd44 antibodies  (ECM Biosciences)
91
ECM Biosciences anti cd44 antibodies
Figure 1: Autocrine OPN effectuates mitochondrial biogenesis via its receptor CD44v. (A) OPN is necessary and sufficient for mitochondrial biogenesis in soft agar colonies. (left and top right, measured as area of fluorescent signal) Transfection with OPNc strongly induced mitochondrial biogenesis as compared to vector. OPNa had an intermediate effect. Addition of anti-OPN antibody O-17 but not control Ig to deadherent cultures of MCF-7 transfectants every other day suppressed the mitochondrial size. (bottom right) Addition of 50%, 75%, 100% supernatants from vector, OPNa or OPNc to deadherent MCF-7 cultures increased the mitochondrial size, with OPNc having a stronger effect than OPNa. (B) Endogenous OPN mediates mitochondrial biogenesis. MDA-MB-435 cells were plated in soft agar. Every other day, they were treated with anti-OPN antibody O-17, anti-OPNc antibody clone 172, control Ig or left untreated (0). Measurements were done either after 5 days for mitochondrial fluorescence (left panel) or after 14 days for colony size (right panel). Fluorescent signal and colony size are expressed as relative units (control = 100 units). Representative photographs are shown in Supplementary Figure 1. (C) The OPN effect on mitochondria and on colony formation is exerted through CD44v. Antibodies to the <t>CD44</t> variable domain, but not to the far N-terminus of CD44 or to Integrin αvβ3, reversed the increase of mitochondrial size (top panels) in soft agar as well as soft agar colony formation (bottom panels) by OPNa-transfected (left bar graphs) and OPNc-transfected (right bar graphs) MCF-7 cells. # = significant increase compared to vector control; * = significant change by treatment (p < 0.05). Fluorescent intensity and colony size are expressed as relative units (control = 100 units). Abbreviation: Sup: supernatant. Representative photographs are shown in Supplementary Figure 2.
Anti Cd44 Antibodies, supplied by ECM Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dedicated+complete+medium+cm-0251/CD44+(Extracellular+region)/pm38039408-182-10-18
Average 91 stars, based on 1 article reviews
anti cd44 antibodies - by Bioz Stars, 2026-10
91/100 stars
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sodium azide crystal violet blood agar  (Thermo Fisher)
98
Thermo Fisher sodium azide crystal violet blood agar
Figure 1: Autocrine OPN effectuates mitochondrial biogenesis via its receptor CD44v. (A) OPN is necessary and sufficient for mitochondrial biogenesis in soft agar colonies. (left and top right, measured as area of fluorescent signal) Transfection with OPNc strongly induced mitochondrial biogenesis as compared to vector. OPNa had an intermediate effect. Addition of anti-OPN antibody O-17 but not control Ig to deadherent cultures of MCF-7 transfectants every other day suppressed the mitochondrial size. (bottom right) Addition of 50%, 75%, 100% supernatants from vector, OPNa or OPNc to deadherent MCF-7 cultures increased the mitochondrial size, with OPNc having a stronger effect than OPNa. (B) Endogenous OPN mediates mitochondrial biogenesis. MDA-MB-435 cells were plated in soft agar. Every other day, they were treated with anti-OPN antibody O-17, anti-OPNc antibody clone 172, control Ig or left untreated (0). Measurements were done either after 5 days for mitochondrial fluorescence (left panel) or after 14 days for colony size (right panel). Fluorescent signal and colony size are expressed as relative units (control = 100 units). Representative photographs are shown in Supplementary Figure 1. (C) The OPN effect on mitochondria and on colony formation is exerted through CD44v. Antibodies to the <t>CD44</t> variable domain, but not to the far N-terminus of CD44 or to Integrin αvβ3, reversed the increase of mitochondrial size (top panels) in soft agar as well as soft agar colony formation (bottom panels) by OPNa-transfected (left bar graphs) and OPNc-transfected (right bar graphs) MCF-7 cells. # = significant increase compared to vector control; * = significant change by treatment (p < 0.05). Fluorescent intensity and colony size are expressed as relative units (control = 100 units). Abbreviation: Sup: supernatant. Representative photographs are shown in Supplementary Figure 2.
Sodium Azide Crystal Violet Blood Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dedicated+complete+medium+cm-0251/Crystal+Violet/pmc10172331-98-10-16
Average 98 stars, based on 1 article reviews
sodium azide crystal violet blood agar - by Bioz Stars, 2026-10
98/100 stars
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capsure hs lcm caps (alignment tray & incubation block required, not included)  (Thermo Fisher)
N/A
Specially designed for use with the ARCTURUS® Laser Capture Microdissection (LCM) instruments, CapSure® HS and CapSure® Macro LCM Caps enable the precise and rapid extraction of pure populations of cells from heterogeneous sample preparations. CapSure
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oxoid diagnostic sensitive test agar  (Thermo Fisher)
N/A
Oxoid Diagnostic Sensitive Test Agar (DSTA) is used for antimicrobial susceptibility testing.
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anti-cd99 (extracellular region) antibody  (PhosphoSolutions)
N/A
Our CD99 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat CD99 (Extracellular region) and is Protein G purified. It is great for use in WB, ICC,
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oxoid crossley milk medium  (Thermo Fisher)
N/A
Examine food samples for anaerobes with Oxoid Crossley Milk Medium, a diagnostic medium.
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oxoid azide blood agar base  (Thermo Fisher)
N/A
Oxoid Azide Blood Agar Base is a selective media for the detection and isolation of streptococci and staphylococci from feces, sewage and other specimens.
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pepyc0cm0210 (plasmid #154542)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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oxoid bismuth sulphite agar (modified wilson & blair medium)  (Thermo Fisher)
N/A
Oxoid Bismuth Sulphite Agar (Modified Wilson & Blair Medium) is used for isolation of Salmonella Typhi and other Salmonella spp., especially lactose-fermenting strains.
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stemxvivo xeno-free human msc expansion media  (R&D Systems)
N/A
StemXVivo Xeno-Free Human MSC Expansion Media
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pepyc0cm0291 (plasmid #154499)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Figure 1: Autocrine OPN effectuates mitochondrial biogenesis via its receptor CD44v. (A) OPN is necessary and sufficient for mitochondrial biogenesis in soft agar colonies. (left and top right, measured as area of fluorescent signal) Transfection with OPNc strongly induced mitochondrial biogenesis as compared to vector. OPNa had an intermediate effect. Addition of anti-OPN antibody O-17 but not control Ig to deadherent cultures of MCF-7 transfectants every other day suppressed the mitochondrial size. (bottom right) Addition of 50%, 75%, 100% supernatants from vector, OPNa or OPNc to deadherent MCF-7 cultures increased the mitochondrial size, with OPNc having a stronger effect than OPNa. (B) Endogenous OPN mediates mitochondrial biogenesis. MDA-MB-435 cells were plated in soft agar. Every other day, they were treated with anti-OPN antibody O-17, anti-OPNc antibody clone 172, control Ig or left untreated (0). Measurements were done either after 5 days for mitochondrial fluorescence (left panel) or after 14 days for colony size (right panel). Fluorescent signal and colony size are expressed as relative units (control = 100 units). Representative photographs are shown in Supplementary Figure 1. (C) The OPN effect on mitochondria and on colony formation is exerted through CD44v. Antibodies to the CD44 variable domain, but not to the far N-terminus of CD44 or to Integrin αvβ3, reversed the increase of mitochondrial size (top panels) in soft agar as well as soft agar colony formation (bottom panels) by OPNa-transfected (left bar graphs) and OPNc-transfected (right bar graphs) MCF-7 cells. # = significant increase compared to vector control; * = significant change by treatment (p < 0.05). Fluorescent intensity and colony size are expressed as relative units (control = 100 units). Abbreviation: Sup: supernatant. Representative photographs are shown in Supplementary Figure 2.

Journal: Oncotarget

Article Title: Osteopontin induces mitochondrial biogenesis in deadherent cancer cells.

doi: 10.18632/oncotarget.28540

Figure Lengend Snippet: Figure 1: Autocrine OPN effectuates mitochondrial biogenesis via its receptor CD44v. (A) OPN is necessary and sufficient for mitochondrial biogenesis in soft agar colonies. (left and top right, measured as area of fluorescent signal) Transfection with OPNc strongly induced mitochondrial biogenesis as compared to vector. OPNa had an intermediate effect. Addition of anti-OPN antibody O-17 but not control Ig to deadherent cultures of MCF-7 transfectants every other day suppressed the mitochondrial size. (bottom right) Addition of 50%, 75%, 100% supernatants from vector, OPNa or OPNc to deadherent MCF-7 cultures increased the mitochondrial size, with OPNc having a stronger effect than OPNa. (B) Endogenous OPN mediates mitochondrial biogenesis. MDA-MB-435 cells were plated in soft agar. Every other day, they were treated with anti-OPN antibody O-17, anti-OPNc antibody clone 172, control Ig or left untreated (0). Measurements were done either after 5 days for mitochondrial fluorescence (left panel) or after 14 days for colony size (right panel). Fluorescent signal and colony size are expressed as relative units (control = 100 units). Representative photographs are shown in Supplementary Figure 1. (C) The OPN effect on mitochondria and on colony formation is exerted through CD44v. Antibodies to the CD44 variable domain, but not to the far N-terminus of CD44 or to Integrin αvβ3, reversed the increase of mitochondrial size (top panels) in soft agar as well as soft agar colony formation (bottom panels) by OPNa-transfected (left bar graphs) and OPNc-transfected (right bar graphs) MCF-7 cells. # = significant increase compared to vector control; * = significant change by treatment (p < 0.05). Fluorescent intensity and colony size are expressed as relative units (control = 100 units). Abbreviation: Sup: supernatant. Representative photographs are shown in Supplementary Figure 2.

Article Snippet: The anti-panOsteopontin antibody O-17 came from Assay Designs Inc., the anti-CD44 antibodies (CM0071, CM0241, CM5911) were provided by ECM Biosciences, the antiIntegrin αv β3 antibody was obtained from R&D Systems.

Techniques: Transfection, Plasmid Preparation, Control, Fluorescence

Figure 6: Model for mitochondrial activation and biogenesis. Immediately after deadhesion, mitochondrial energy production is enhanced, with OPNa increasing the cellular glucose uptake and OPNc utilizing this glucose for mitochondrial ATP production (left). Over extended timeframes of deadhesion, the elevated energy requirement is met through mitochondrial biogenesis. OPNc ligates CD44v and SLC7A11 to generate peroxides, induce PGC-1 activity and increase mitochondrial size. OPNa only ligates CD44 (not SLC7A11) and exerts a minor effect on mitochondrial biogenesis (right). The probes employed are shown in Supplementary Figure 6.

Journal: Oncotarget

Article Title: Osteopontin induces mitochondrial biogenesis in deadherent cancer cells.

doi: 10.18632/oncotarget.28540

Figure Lengend Snippet: Figure 6: Model for mitochondrial activation and biogenesis. Immediately after deadhesion, mitochondrial energy production is enhanced, with OPNa increasing the cellular glucose uptake and OPNc utilizing this glucose for mitochondrial ATP production (left). Over extended timeframes of deadhesion, the elevated energy requirement is met through mitochondrial biogenesis. OPNc ligates CD44v and SLC7A11 to generate peroxides, induce PGC-1 activity and increase mitochondrial size. OPNa only ligates CD44 (not SLC7A11) and exerts a minor effect on mitochondrial biogenesis (right). The probes employed are shown in Supplementary Figure 6.

Article Snippet: The anti-panOsteopontin antibody O-17 came from Assay Designs Inc., the anti-CD44 antibodies (CM0071, CM0241, CM5911) were provided by ECM Biosciences, the antiIntegrin αv β3 antibody was obtained from R&D Systems.

Techniques: Activation Assay, Activity Assay